_leukocyte-rolling-on-p-selectin-followed-by-activation-induced-arrest-on-icam-1-in-vitro.jpg "Leukocyte rolling on P-selectin followed by activation-induced arrest on ICAM-1 in vitro from icam Video Screenshot Preview")
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⏲ Duration: 39 sec
✓ Published: 14-Oct-2015
✓ Published: 14-Oct-2015
Description:
All three steps can be reconstituted in vitro. Both P-selectin and ICAM-1 are incorporated on the wall of a flow chamber. Neutrophils are infused and begin rolling on the P-selectin. There is no interaction with ICAM-1, because the aMb2 and aLb2 integrins on the neutrophil have not been activated. Subsequently, a chemoattractant such as a bacterial N-formylated peptide (fMLP) is added to the perfusate. When it reaches the position of the rolling neutrophils in the flow chamber, it rapidly activa
All three steps can be reconstituted in vitro. Both P-selectin and ICAM-1 are incorporated on the wall of a flow chamber. Neutrophils are infused and begin rolling on the P-selectin. There is no interaction with ICAM-1, because the aMb2 and aLb2 integrins on the neutrophil have not been activated. Subsequently, a chemoattractant such as a bacterial N-formylated peptide (fMLP) is added to the perfusate. When it reaches the position of the rolling neutrophils in the flow chamber, it rapidly activa
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